Eyestalk ablation is the most common procedure to induce gonadic maturation of Eriocheir sinensis. In addition to reproduction, other physiological and metabolic processes are also affected by the removal of X-organ sinus complex located in the eyestalk. In this study, we studied physiological and biochemical effects of eyestalk ablation of E. sinensis, and the results were as follows. The amylase activity of male crabs with unilateral or bilateral eyestalk ablaion was increased with the days increasing, and the maximal value was on the 20th day (P P