Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i>oleaginous type

• 作者: Irié Arsène Zoro Bi Jean-Pierre Baudoin Yah Gwladys Gnamien Yatty Justin Kouadio Yves Brostaux
• 来源期刊: 农业科学(英文)
• 学科: 医学
• 关键词: AFLP Genetic Stability In VITRO Oleaginous SEEDS ORGANOGENESIS
• 年，卷（期）: 2013,（7）
• 页码范围: 32-44
• 页数: 13页
• 分类号: R73

摘要

To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.