Green recycling of chicken feather and sheep wool using the partially purified alkaline protease from <i>Bacillus circulans</i>L.

• 作者: Eman Fathi Sharaf Khadijah Al-Fadel
• 来源期刊: 自然科学期刊(英文)
• 学科: 医学
• 关键词: Bacillus Alkaline PROTEASE Ammonium Sulphate Precipitation Characterization FEATHER WOOL Blood STAINS
• 年，卷（期）: 2013,（6）
• 页码范围: 25-29
• 页数: 5页
• 分类号: R73

摘要

The previously optimized crude alkaline protease from the haloalkaliphilic Bacillus circulans L. was partially purified using ammonium sulphate fractionation and dialysis. The best specific activity (27.7 U/mg protein) was obtained at 80% saturation. The optimum reaction temperature and reaction pH was 47℃ and 9, respectively. The enzyme activity was enhanced with Ca and K chlorides but suppressed with HgCl2 and EDTA. The partially purified protease showed strong proteolytic activity on sheep wool and chicken feather. Also, the enzyme was compatible with the common detergent Tide and could improve its cleaning power in removing blood stain. These findings support the application of the present alka-line protease in biotechnological industries.