Quantification of Solid Residues by Raman Spectroscopy

• 作者: América Vázquez-Olmos Esau Araiza-Reyna Roberto Sato-Berrú
• 来源期刊: 光学与光子学期刊(英文)
• 学科: 医学
• 关键词: Quantitative RAMAN AMINOACIDS GLYCINE SOLID RESIDUES Drop Coating Deposition RAMAN Principal Component Analysis
• 年，卷（期）: 2013,（5）
• 页码范围: 1-6
• 页数: 6页
• 分类号: R73

摘要

We suggest a mathematical route which has the capability to obtain quantitative information of solid residues on a substrate of aluminum, in which the evaporation of the deposited micro-drops has the form of coffee rings. In this job, the glycine aminoacid was used as probe molecule. We suppose that the number of moles present on the study sample is proportional to the Raman signal and to the experimental parameters of the used Raman system. Then, the mass and the number of molecules can be determined with these data. We showed that the mathematical expression is simple and elegant for its implementation. On the other hand, we have applied and demonstrated that the technique of principal component analysis (PCA) can be used to obtain quantitative information of the sample. Our results show quantitative analysis of glycine residues between 10-11 to 10-15 g.