Identification of Phenotypic and Genotypic Variability among the Isolates of <i>Ramularia areola</i>of Brazilian Cotton

• 作者: Janaina N. Matos Larissa Girotto Mariana S. Marangoni Rafael Galbieri Wilson P. Almeida Yeshwant R. Mehta
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Ramularia False MILDEW GENOTYPIC and PHENOTYPIC Differentiation Gossypium hirsutum
• 年，卷（期）: 2013,（9）
• 页码范围: 1893-1898
• 页数: 6页
• 分类号: R73

摘要

Genotypic and phenotypic variation among 16 isolates of Ramularia areola of Gossypium hirsutum collected from five different geographical regions of Brazil was studied through virulence spectrum on three cultivars in the glasshouse and through ERIC- and REP-PCR and ITS1-5.8S-ITS2 rDNA analysis. Difference in virulence spectrum and molecular analysis of some isolates was observed. ERIC- and REP-PCR showed similar results and revealed a high level of diversity among the isolates. A unique profile for both ERIC and REP was obtained for most isolates. On the other hand, the ITS rDNA analysis did not show different PCR-RFLP patterns. While some isolates differed among each other considering genotypic and phenotypic reactions, no clear cut evidence was found about the existence of genetic lineages of R. areola in Brazil. Identification of genetic variability among the R. areola isolates originated from different geographic regions would permit screening of Brazilian germplasm and achieve sources with a wide spectrum of resistance. This is the first report of the genotypic and phenotypic variability among the R. areola isolates originated from five cotton growing regions of Brazil.