Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification

• 作者: Andrea E. Holmes Gary Batres Hannah Johnke Mark Wilson Sharmin Sikich Talia Jones
• 来源期刊: 传感技术(英文)
• 学科: 医学
• 关键词: COLORIMETRIC ARRAYS Sensors Color Changes STEROIDS Metabolic Disease METABOLITES Detection
• 年，卷（期）: 2014,（1）
• 页码范围: 1-6
• 页数: 6页
• 分类号: R73

摘要

The work described herein examines a rapid mix-and-measure method called DETECHIP suitable for screening of steroids and metabolites. The addition of steroids and metabolites to reactive arrays of colorimetric sensors generated characteristic color “fingerprints” that were used to identify the analyte. A color analysis tool was used to identify the analyte pool that now includes biologically relevant analytes. The mix-and-measure arrays allowed the detection of disease metabolites, orotic acid and argininosuccinic acid;and the steroids androsterone, 1,4-androstadiene, testosterone, stanozolol, and estrone. The steroid 1,4-androstadiene was also detected by this method while dissolved in synthetic urine. Some of the steroids, such as androstadiene, stanozolol, and androsterone were co-dissolved with (2-hydroxypropyl)-β-cyclodextrin in order to increase solubility in aqueous buffered solutions. The colorimetric arrays do not intend to eliminate ELISA or mass spectroscopy based screening, but to possibly provide an alternative analytical detection method for steroids and metabolites.