Nuclear factorκB represses the expression of latent membrane protein 1 in Epstein-Barr virus transformed cells

• 作者: Amy Lingel Luwen Zhang Mingxia Cao Qianli Wang
• 来源期刊: 世界病毒学杂志
• 学科: 医学
• 关键词: NUCLEAR factorκB EPSTEIN-BARR virus LATENT membrane protein 1 LATENCY Transformation
• 年，卷（期）: 2014,（4）
• 页码范围: 22-29
• 页数: 8页
• 分类号: R373

摘要

AIM:To investigate the role of nuclear factorκB(NF-κB)in the regulation of Epstein-Barr virus(EBV)latent membrane protein 1(LMP1)in EBV transformed cells.METHODS:LMP1 expression was examined in EBV transformed human B lymphocytes with modulation of NF-κB activity.RESULTS:EBV infection is associated with several human cancers.EBV LMP1 is required for efficient transformation of adult primary B cells in vitro,and is expressed in several pathogenic stages of EBVassociated cancers.Regulation of EBV LMP1 involves both viral and cellular factors.LMP1 activates NF-κB signaling pathway that is a part of the EBV transformation program.However,the relation between NF-κB and LMP1 expression is not well established yet.In this report,we found that blocking the NF-κB activity by Inhibitor ofκB stimulated LMP1 expression,while the overexpression of NF-κB repressed LMP1 expression in EBV-transformed IB4 cells.In addition,LMP1 repressed its own promoter activities in reporter assays,and the repression was associated with the activation of NF-κB.Moreover,NF-κB alone is sufficient to repress LMP1 promoter activities.CONCLUSION:Our data suggest LMP1 may repress its own expression through NF-κB in EBV transformed cells and shed a light on LMP1 regulation during EBV transformation.