Electrospun Gelatin Constructs with Tunable Fiber Orientation Promote Directed Angiogenesis

• 作者: Fotios M. Andreopoulos Gianluca D’Ippolito Ramon B. Montero Roberto I. Vazquez-Padron Si M. Pham
• 来源期刊: 再生医学期刊(英文)
• 学科: 医学
• 关键词: Electrospinning bFGF Directed ANGIOGENESIS Vessel Formation Critical LIMB Ischemia SCAFFOLD Fabrication
• 年，卷（期）: 2014,（1）
• 页码范围: 1-12
• 页数: 12页
• 分类号: R73

摘要

The field of therapeutic angiogenesis has been predominantly concentrated in modalities that incorporate pro-angiogenic growth factors and/or cells within polymeric constructs that are implanted into the ischemic region. There is growing evidence that construct architecture can significantly affect growth factor activity, cellular viability and differentiation potential. Electrospinning is an attractive but simple scaffold fabrication technique that offers several advantages over traditional fabrication approaches to prepare highly organized structures for therapeutic angiogenesis applications. We recently described the fabrication of nanofibrous scaffolds with aligned fiber orientation that directed cell migration and orientation (i.e.human umbilical vein endothelial cells). Herein we demonstrate the ability of bFGF containing nanofibrous gelatin B scaffolds with controlled fiber orientation to promote capillary formation in vivo. Aligned scaffolds loaded with bFGF induced the highest levels of reperfusion (73% increased in LDPI ratios by day 21 post ischemia induction) in comparison to all other groups including scaffolds with random fiber orientation. Furthermore, the newly formed vasculature, assessed by confocal microscopy, had a parallel alignment along the axis of the scaffold’s fibers. In contrast, no vessel directionality was observed in the animals treated with scaffolds with random fiber orientation in the presence or absence of bFGF.