Preparation of Microcapsules Containing Aqueous Solution of Azur B with Melting Dispersion Cooling Method and Application to DNA Amplification Detector

• 作者: Masato Tanaka Natsukaze Saito Ryohei Yamamoto Yoshinari Taguchi
• 来源期刊: 封装与吸附期刊(英文)
• 学科: 化学
• 关键词: MICROCAPSULES AZUR B DNA Amplification DETECTOR MELTING DISPERSION Cooling Method Multiple EMULSION
• 年，卷（期）: 2014,（1）
• 页码范围: 15-24
• 页数: 10页
• 分类号: O6

摘要

Microcapsules containing the aqueous solution of Azur B of a water soluble dye were prepared with the melting dispersion cooling method and applied to the amplification detector of plant DNA. Paraffin wax with melting temperature of 75°C was used as the shell material. In the experiment, the aqueous solution (W) of Azur B as the core material was dispersed in the melted paraffin wax (O) to form the (W/O) emulsion and then, the (W/O) emulsion was dispersed in the silicon oil (O’) as the continuous phase to form the (W/O)/O’ emulsion at 85°C. After formation of the (W/O)/O’ emulsion, the microcapsules were prepared by cooling the (W/O)/O’ emulsion to 50°C. The microcapsules were prepared by changing the concentration of oil soluble surfactant in the (W/O) emulsion and the volume of the (W/O) emulsion in the (W/O)/O’ emulsion. The microencapsulation efficiency increased with the concentration of oil soluble surfactant and finally became 100% under the optimum conditions. Furthermore, the microcapsules were melted down at temperature of 85°C to reveal the sharp thermal responsibility and to release the aqueous solution of Azur B. As a result, it was found that the microcapsules were able to be applied to the amplification detector of plant DNA by utilizing the reaction between DNA and Azur B.