GenoType MTBDR<i>plus</i>as a Complementary Tool for the Typing of <i>Mycobacterium tuberculosis</i>

• 作者: Guillaume Yao Loukou Hortense Faye-Kette Timothée Ouassa
• 来源期刊: 传染病进展(英文)
• 学科: 医学
• 关键词: MYCOBACTERIUM tuberculosis MIRU-VNTR SPOLIGOTYPING GENOTYPE MTBDRplus C?te d’Ivoire
• 年，卷（期）: 2014,（1）
• 页码范围: 26-29
• 页数: 4页
• 分类号: R73

摘要

The aim of this study was to investigate the usefulness of combining profiles obtained by using a line probe assay (LPA) originally intended to characterize the resistance of two major anti-tuberculosis drugs to the association of spoligotyping and MIRU-VNTR, in order to improve its discriminatory power. For this purpose, 74 strains of Mycobacterium tuberculosis belonging to the same cluster after spoligotyping were further typed by using the 24 loci MIRU/VNTR. These strains were then tested by the GenoType MTBDRplus, and profiles obtained were analyzed within previously obtained clusters. The combination of spoligotying and MIRU-VNTR led to the consolidation of 56 of them (75.7%) in 9 clusters. Most of the strains (54, 96.4%) were multidrug resistant (MDR). From the 9 initial clusters, the addition of GenoType MTBDRplus helped to define 26 profiles including 11 unique profiles, and 3 original clusters remained undifferentiated. Results obtained express the relevance of combining this method which improved quite significantly the discriminatory power in typing Mycobacterium tuberculosis.