Crocetin Prevents Amyloid <i>β</i><sub>1-42</sub>-Induced Cell Death in Murine Hippocampal Cells

• 作者: Hideaki Hara Kazuhiro Tsuruma Masamitsu Shimazawa Mitsue Ishisaka Naofumi Umigai Yuta Yoshino
• 来源期刊: 药理与制药(英文)
• 学科: 医学
• 关键词: Alzheimer’s Disease AMYLOID β1-42 CROCETIN HT22 Oxidative Stress
• 年，卷（期）: 2014,（1）
• 页码范围: 37-42
• 页数: 6页
• 分类号: R28

摘要

Crocetin is an aglycon of carotenoid extracted by saffron stigmas (Crocus sativus L.) and known to have a potent anti-oxidative effect. Amyliod β (Aβ), hallmark of Alzheimer’s disease, is reported to have neurotoxicity partly via oxidative stress. In this study, we investigated the effect of crocetin on hippocampal HT22 cell death induced by Aβ1-42. Furthermore, to clarify the mechanism underlying the protective effects of crocetin against Aβ1-42- induced cell death, we measured reactive oxygen species (ROS) production by CM-H2DCFDA kit assay. Crocetin at 1 -10 μM protected HT22 cells against Aβ1-42-induced neuronal cell death and decreased ROS production increased by Aβ1-42. These results that crocetin has the potent neuroprotective effect against Aβ1-42-induced cytotoxicity in hippocampal cells by attenuating oxidative stress, suggest that crocetin may provide a useful therapeutic strategy against Aβ-related disorders.