Optimization of Parameters for the Production of Lipase from Pseudomonas sp. BUP6 by Solid State Fermentation

• 作者: Erandapurthukadumana Sreedharan Hareesh Kizhakkepowathial Nair Unni Moolakkariyil Sarath Josh Panichikkal Abdul Faisal Prakasan Priji Sailas Benjamin Sasidharan Sreedevi Sreedharan Sajith
• 来源期刊: 酶研究进展(英文)
• 学科: 医学
• 关键词: LIPASE SOLID-STATE FERMENTATION BASAL SALT Medium Oil CAKES Response Surface Methodology
• 年，卷（期）: 2014,（4）
• 页码范围: 125-133
• 页数: 9页
• 分类号: R73

摘要

Solid-state fermentation (SSF) holds tremendous potentials for the production of industrially significant enzymes. The present study describes the production of lipase by a novel rumen bacterium, Pseudomonas sp. strain BUP6 on agro-industrial residues. Pseudomonas sp. strain BUP6 showed higher lipase production when grown in Basal salt medium (BSM) supplemented with oil cakes. Initially, five different oil cakes (obtained after extracting oil from coconut, groundnut, cotton seed, gingelly or soybean) were screened to find out the most suitable substrate-cum-inducer for the production of lipase. Among them, groundnut cake supported the maximum production of lipase (107.44 U/gds). Box-Behnken Design (BBD), followed by response surface methodology (RSM) was employed to optimize the culture parameters for maximizing the production of lipase. Using the software Minitab 14, four different parameters like temperature, pH, moisture content and incubation time were selected for the statistical optimization, which resulted in 0.7 fold increase (i.e., 180.75 U/gds) in production of lipase under the optimum culture conditions (temperature 28&#176C, pH 5.9, moisture 33% and incubation 2 d). Thus, this study signifies the importance of SSF for the production of industrially-significant lipase using agro-industrial residues as solid support.