Value of a newly sequenced bacterial genome

• 作者: Adriana R Carneiro Anderson Miyoshi Artur Silva Eudes GV Barbosa Flavia F Aburjaile Jan Baumbach Rommel TJ Ramos Vasco Azevedo Yves Le Loir
• 来源期刊: 世界生物化学杂志：英文版（电子版）
• 学科: 生物学
• 关键词: Next-generation sequencing DRAFTS PROKARYOTIC GENOMES Computational tools OMICS
• 年，卷（期）: 2014,（2）
• 页码范围: 161-168
• 页数: 8页
• 分类号: Q75

摘要

Next-generation sequencing(NGS) technologies have made high-throughput sequencing available to medium- and small-size laboratories, culminating in a tidal wave of genomic information. The quantity of sequenced bacterial genomes has not only brought excitement to the field of genomics but also heightened expectations that NGS would boost antibacterial discovery and vaccine development. Although many possible drug and vaccine targets have been discovered, the success rate of genome-based analysis has remained below expectations. Furthermore, NGS has had consequences for genome quality, resulting in an exponential increase in draft(partial data) genome deposits in public databases. If no further interests are expressed for a particular bacterial genome, it is more likely that the sequencing of its genome will be limited to a draft stage, and the painstaking tasks of completing the sequencing of its genome and annotation will not be undertaken. It is important to know what is lost when we settle for a draft genome and to determine the 'scientific value' of a newly sequenced genome. This review addresses the expected impact of newly sequenced genomes on antibacterial discovery and vaccinology. Also, it discusses the factors that could be leading to the increase in the number of draft deposits and the consequent loss of relevant biological information.