Impact of Different Carbohydrates and Their Concentrations on <i>in Vitro</i>Regeneration of <i>Solanum viarum</i>(Dunal)—An Important Anticancer Medicinal Plant

• 作者: ChallagundlaVaradarajulu Naidu Chandra Sekhar Panathula Manikyam Doraiswamy Naidu Mahadev
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: SOLANUM viarum Carbohydrates Plant REGENERATION Multiple SHOOT Induction
• 年，卷（期）: 2014,（1）
• 页码范围: 200-204
• 页数: 5页
• 分类号: R73

摘要

In this present study, the effect of various carbon sources such as sucrose, glucose, fructose and maltose was investigated on in vitro shoot regeneration of Solanum viarum using axillary bud explants. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon source used. Among the different concentrations (1%-6%) of carbohydrates studied, the maximum number of shoots (22.6 ± 0.50) and shoot length (5.92 ± 0.13 cm) was obtained on MS medium supplemented with 4% (w/v) fructose. The least number of shoots (1.5 ± 0.32) was obtained on 6% maltose and the least shoot length (1.2 ± 0.23) was observed on 6% glucose. Among the four types of carbon sources that were employed in the present study, fructose at 4% proved to be better choice for multiple shoot regeneration followed by sucrose, glucose and maltose from axillary bud explants of S. viarum.