Development of a Genetic Transformation Method for Seabuckthorn (<i>Hippophae rhamnoides</i>L.)

• 作者: David Clapham Per-Olof Lundquist Sridevy Sriskandarajah
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: HIPPOPHAE rhamnoides Particle BOMBARDMENT Transformation
• 年，卷（期）: mgzwxqkyw_2014,（5）
• 页码范围: 528-534
• 页数: 7页
• 分类号: R73

摘要

Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing high amounts of several bioactive compounds with nutritional and medicinal traits. It is also planted to control soil erosion. A genetic transformation procedure will facilitate studies of the control of plant development and interactions with symbionts and pathogens, and will provide a tool for plant breeding. Here, we present a particle bombardment method for transforming seabuckthorn. The early stages of induced adventitious shoots from roots were chosen as a novel target tissue for the transformation procedure. The root system was bombarded with gold particles coated with plasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression, and shoots were regenerated under kanamycin selection. PCR analysis of the regenerated transformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kb fragment from the 35S promoter in three shoots from independent transformation events.