Optimum Protocol for Shoot Formation in karandafi Red Sorghum (<i>Sorghum bicolor</i>(L.) Moench) through Somatic Embryogenesis Using Mature Embryo

• 作者: Inuwa S. Usman Lateefat Bolanle Hassan Mary D. Katung Sanusi M. Bugaje
• 来源期刊: 美国植物学期刊(英文)
• 学科: 农学
• 关键词: SORGHUM BICOLOR CALLUS Induction In-Vitro Regeneration
• 年，卷（期）: 2014,（5）
• 页码范围: 671-675
• 页数: 5页
• 分类号: S5

摘要

In this study, an optimum protocol for shoot formation through somatic embryogenesis using mature embryo explants was developed. Calli were initiated on Murashige and Skoog (MS) media supplemented with varying concentration of 2,4-Dichlorophenoxyl acetic acid (2,4-D) ranging from 1.5 mg/l-4.0 mg/l alone or in combination with 0.5 mg/l Kinetin (KN). Significance difference (p 0.05) was observed among the different concentrations of hormone used for callus induction. The highest percentage callus formation was obtained from the media fortified with 4 mg/l 2, 4-D for mature embryo obtained from imbibed seed while for the preconditioned mature embryo, the media supplemented with 2 m/l 2,4-D + 0.5 mg/l kinetin (KN) recorded more percentage callus formation compared to what was obtained from the media supplemented with 2.5 mg/l2,4-D + 0.5 mg/l. More percentage shoot formation was obtained from the media supplemented with 1 mg/l 6-benzylaminopurine (BA). Average number of shoot per callus was also more in the media fortified with 1 mg/l BA (2.25) but this was not significantly different from what was obtained from the media fortified with 2 mg/l BA+ 0.1 mg/l 2,4-D at 5% level of significance.