Rubber Seed Kernel as Potent Solid Substrate for the Production of Lipase by Pseudomonas aeruginosa Strain BUP2

• 作者: E. S. Hareesh K. N. Unni Panichikkal Abdul Faisal Prakasan Priji Sailas Benjamin Sreedharan Sajith Sreedharan Sreedevi Trikariyoor Asokan Nidheesh Roy
• 来源期刊: 酶研究进展(英文)
• 学科: 医学
• 关键词: PSEUDOMONAS AERUGINOSA BUP2 LIPASE Solid-State Fermentation BUP Medium RUBBER Seed FLOUR
• 年，卷（期）: 2015,（2）
• 页码范围: 31-38
• 页数: 8页
• 分类号: R73

摘要

This study explored the utility of flours of rubber seed, coconut and groundnut kernels, and de-oiled cakes of coconut and groundnut as solid substrate for the production of lipase by Pseudomonas aeruginosa strain BUP2 (MTCC No. 5924), a novel bacterium reported from the rumen of Malabari goat. Various proportions (10%, 20%, 30%, 40% or 50%) of flours or cakes were prepared (w/v) with BUP medium (pH 4, 5, 6, 7 or 8), and incubated at different temperature (25°C, 28°C, 30°C or 32°C) for 24 to 96 h. The samples were assayed for lipase activity at 24 h intervals. The rubber seed flour (20%)-BUP medium supported the maximum lipase production (871 U/gds) at 48h incubation (pH 6, 28°C), followed by ground nut flour (398 U/gds), while ground nut cake supported the least lipase production (244 U/gds). From this, it is evident that the cheaply available rubber seed is an efficient substrate for the production of lipase, irrespective of its known demerit that it contains the limarin, a toxin;in fact, we could not detect limarin in the fermented matter. Thus, the utility of rubber seed for the production of a costly enzyme is reported from a novel rumen bacterium, which would be advantageous for rubber farmers.