Development of RAPD-based SCAR Marker for Rapid Identification of Trichoderrna harzianurn

• 作者: Li Li Su Zhenyu Sun Yuantian Tang Libo
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: SCAR标记 RAPD分析 快速鉴定 特异性引物 基因组DNA 木霉菌株 开发 RAPD扩增
• 年，卷（期）: pdapzwbchyj,（2）
• 页码范围: 12-15
• 页数: 4页
• 分类号: S512.1

摘要

Based on previous morphological classification and RAPD analysis,we recovered and sequenced genomic DNA of a specific fragment obtained from No.45 Trichoderma harzianumstrain in RAPD amplification.A pair of specific primers was synthesized and the RAPD markers were successfully converted into SCAR markers.The SCAR molecular marker exhibited the specificity that could differ T.harzianumfrom other Trichoderma strains.This pair of molecular primers was used for specific PCR amplification of 45 Trichoderma strains,and T.harzianumstrains could amplify a 779 bp DNA band while other strains had no amplification products.