Molecular Characterization of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Gene of Field Isolate of <i>Edwardsiella tarda</i>

• 作者: Gayatri Tripathi K. V. Rajendran M. Makesh Megha K. Bedekar Praveena K. Soman
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 医学
• 关键词: E. tarda GAPDH GENE Vaccine Cloning
• 年，卷（期）: smkxyjsjzyw,（2）
• 页码范围: 89-95
• 页数: 7页
• 分类号: R73

摘要

The E. tarda bacterial culture isolated from infected fish, was confirmed by morphological and biochemical characterization. GAPDH gene of 996 bp was amplified from bacterial genomic DNA. GAPDH gene was cloned in pTZ57R/T cloning vector. The positive clone was sequenced. The sequencing result showed very homology with published sequence from pathogenic E. tarda. The sequence (nucleotide and amino acid) divergence values were very low between E. tarda isolates from India (KF142190) and China (HQ697337-38) (Figure 4 and Figure 5). However, the divergence value was high when compared with E. tarda isolates from Japan (AB198939) and this value was higher than the inter-specific divergence value (E. tarda-E. ictaluri, E. tarda-E. coli & E. tarda-Klebsiella species).