Direct Shoot Regeneration from Callus of <i>Melicope lunu-ankenda</i>

• 作者: Ab. Zuraida Rahman Ayu Nazreena Othman Aziz Bin Ahmad Fatin Liyana Izzati Kamaruddin
• 来源期刊: 自然科学期刊(英文)
• 学科: 医学
• 关键词: Melicope lunu-ankenda REGENERATION Plant Growth REGULATOR In VITRO
• 年，卷（期）: zrkxqkyw,（2）
• 页码范围: 81-87
• 页数: 7页
• 分类号: R73

摘要

Melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 - 5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.