High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i>Follicle Stimulation

• 作者: Geralda Walkiria de Araujo Ivan Carlos dos Santos Jorge Luiz Pesquero Meire Coelho de Almeida Mercia de Paula Lima Nélia Ferreira de Souza Raquel Mariana de Melo Leite
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 医学
• 关键词: RECOMBINANT HUMAN ACTIVIN Yeast SUPEROVULATION
• 年，卷（期）: 2015,（2）
• 页码范围: 96-104
• 页数: 9页
• 分类号: R73

摘要

Activin plays an important role in numerous physiological processes such as cell differentiation and remodeling, regeneration and repair of tissues from various organs, angiogenesis, morphogenesis of glandular organs, pluripotency and differentiation of stem cells, cell adhesion and apoptosis. It participates in reproductive processes like embryogenesis, in the expression of Follicle Stimulating Hormone and Luteinizing Hormone and maturation of ovarian follicles and therefore has application in the area of reproduction of vertebrates. Given the economic importance of activin, we develop an efficient and economical method for the production of recombinant human activin A (rACT), using as expression system the yeast Pichia pastoris. rACT showed biological activity as it induced, on submicromolar dose, the increase of ovarian mass and the ovulation process in a mammal model.