Screening and Identification of Chitinolytic Actinomycetes and Study on the Inhibitory Activity against Turfgrass Root Rot Disease Fungi

• 作者: Bihua Duan Lu Zhang Qi Wang Rui Yang Yaorong Zhao
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: Strain Bn035 TRANSPARENT Circle CHITINASE Activity ANTAGONISM 16SrDNA
• 年，卷（期）: 2015,（3）
• 页码范围: 56-65
• 页数: 10页
• 分类号: R73

摘要

Objective: This study aimed to separate and pure chitinase producing actinomycetes with stronger activity from lawn soils of Beijing. Method: Morphology and culture characteristics were observed. physiological and biochemical characteristics were detected. 16SrDNA sequence was analyzed. Result: The highest chitinase activity strain was Bn035, whose relative diameter of transparent circle K2 was 3.42 at which the chitinase activity of fermentation broth reached a maximum value of 1.23 U/mL in the 6th day. Crude enzyme solution and bacteria cake of strain Bn035 generated stronger bacterial resistance to all root rot fungi which indicated that the strain had a strong broad-spectrum resistance to root rot fungi. Conclusion: The strain was identified as Streptomyces scabrisporus. It will be a potential biological control function.