Phylogenetic Analysis and Taste Cell Expression of Calpain 9 in Catfish (<i>Ictalurus punctatus</i>)

• 作者: Anne Hansen Eiki Koyama John H. Teeter Joseph G. Brand Tetsuya Ookura Yukio Kawamura
• 来源期刊: 自然科学期刊(英文)
• 学科: 医学
• 关键词: Gene Evolution TASTE Novel Type
• 年，卷（期）: zrkxqkyw_2015,（3）
• 页码范围: 143-150
• 页数: 8页
• 分类号: R73

摘要

The calpains, calcium-activated neutral proteases, play important roles in calcium-regulated intra-cellular signal transduction cascades. Here we report the isolation and initial characterization of a cDNA encoding a calpain 9, digestive tract specific calpain, from catfish taste epithelium. This calpain 9 (Ip-CAPN9a) shares 61% identity with human calpain 9. Phylogenetic analysis provides evidence that catfish calpain 9 and the related enzymes from Oncorhynchus mykiss, Danio rerio, Xenopus laevis, Mus musculus, Rattus norvegicus and Homo sapiens make up a distinct clade within the tissue-specific calpain family. Northern blot analysis reveals that Ip-CAPN9a is predominantly expressed in barbell and digestive tract, but not expressed in brain. An antibody against the N-terminal segment of Ip-CAPN9a recognizes cells within the taste buds in catfish barbells.