Clinical Evaluation of New Biomarkers including IFN-<i>γ</i>for the Diagnosis of Active Tuberculosis Disease

• 作者: Keiji Mouri Mikio Oka Shigeki Kato Yoshihiro Kobashi
• 来源期刊: 结核病研究(英文)
• 学科: 医学
• 关键词: Mycobacterium TUBERCULOSIS (MTB) DISEASE INTERFERON-Γ (IFN-γ) NEW Biomarkers Interleukin-2 (IL-2)
• 年，卷（期）: 2015,（4）
• 页码范围: 136-148
• 页数: 13页
• 分类号: R73

摘要

Background: The aim of this study was to evaluate the clinical potential of new biomarkers such as IL-2, TNF-α, MCP-1, MIP-1β including IFN-γ for the diagnosis of active tuberculosis (TB) disease. Materials and Methods: The subjects consisted of 31 patients with active TB disease and 62 patients with non-TB disease. We measured IFN-γ using QuantiFERON-TB Gold In Tube, and IL-2, TNF-α, MCP-1, and MIP-1β using the supernatant from whole blood stimulated with MTB (Mycobacterium tuberculosis)-specific antigens. Results: In the patient group with active TB disease, while the positive response rate of IFN-γ was 74%, that of IL-2 using the supernatant was 61%, TNF-α was 71%, MCP-1 was 81% and MIP-1β was 81%. In the patient group with non-TB disease, while the positive response rate of IFN-γ was 11%, that of IL-2using the supernatant was 32%, TNF-α was 26%, MCP-1 was 23% and MIP-1β was 19%. All biomarker levels of the patients with active TB disease using the supernatant were significantly higher than those of the patients with non-TB disease. While MIP-1β was the most sensitive of all the biomarkers (sensitivity: 80.6%), IFN-γ was the best in terms of specificity (specificity: 87.0%). Conclusions: Several biomarkers, apart from IL-2, showed similar results compared to IFN-γ. The combination of IFN-γ and other new biomarkers may increase the diagnostic accuracy of active TB.