Radiation signature on exposed cells: Relevance in dose estimation

• 作者: Amith Roy Chowdhury Karthik Kanagaraj Safa Abdul Syed Basheerudeen Solomon FD Paul Tamizh Selvan Gnana Sekaran Venkatachalam Perumal Venkateswarlu Raavi
• 来源期刊: 世界放射学杂志：英文版（电子版）
• 学科: 医学
• 关键词: BIOMARKER Dicentric chromosomes MICRONUCLEUS FLUOR
• 年，卷（期）: 2015,（9）
• 页码范围: 266-278
• 页数: 13页
• 分类号: R811.5

摘要

The radiation is considered as a double edged sword, as its beneficial and detrimental effects have been demonstrated. The potential benefits are being exploited to its maximum by adopting safe handling of radionuclide stipulated by the regulatory agencies. While the occupational workers are monitored by personnel monitoring devices, for general publics, it is not a regular practice. However, it can be achieved by using biomarkers with a potential for the radiation triage and medical management. An ideal biomarker to adopt in those situations should be rapid, specific, sensitive, reproducible, and able to categorize the nature of exposure and could provide a reliable dose estimation irrespective of the time of the exposures. Since cytogenetic markers shown to have many advantages relatively than other markers, the origins of various chromosomal abnormalities induced by ionizing radiations along with dose-response curves generated in the laboratory are presented. Current status of the gold standard dicentric chromosome assay, micronucleus assay, translocation measurement by fluorescence insitu hybridization and an emerging protein marker the g-H2 AX assay are discussed with our laboratory data. With the wide choice of methods, an appropriate assay can be employed based on the net.