Optimization of Mycelial Biomass Production in Submerged Culture Fermentation of <i>Pleurotus flabellatus</i>Using Response Surface Methodology

• 作者: Aidil Abdul Hamid Fauzi Daud Liew Siew Ling Mat Rasol Awang Rahayu Ahmad Rosnani Abdul Rashid Shaiful Azuar Mohamad Wan Mohtar Wan Yusoff
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 医学
• 关键词: Submerged Culture FERMENTATION Response Surface Methodology MUSHROOM PRODUCTION
• 年，卷（期）: 2015,（6）
• 页码范围: 419-426
• 页数: 8页
• 分类号: R73

摘要

The factors selected to optimize the productivity of Pleurotus flabellatus biomass in 250ml working volume Erlenmeyer flask were agitation rate, initial pH value and incubation temperature. The central composite design was applied to study the significant factors and the interactions between the chosen factors, if present. The Design Expert software generated 20 runs. The optimized conditions obtained were as follows: the agitation rate of 129.8 rpm, incubation temperature at 27.8&degC, and initial pH of 6.06. The optimized conditions tripled the productivity at the range of 980 - 1040 mg/litre/day compared to the initial rate productivity at 310 mg/litre/day. From the quadratic equation,the agitation rate, temperature and the interaction between agitation rate and temperature were found to be significant (p < 0.05). At optimum conditions, the experimental data supported the theoretical estimate.