Comparison of pre-treatment and post-treatment use of selenium in retinal ischemia reperfusion injury

• 作者: Alper Yazici Arzu Yay Dilek Aksit Esin Sogutlu Sari Harun Cakmak Hasan Aksit Haydar Ali Erken Kamil Seyrek Sitki Samet Ermis
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: RETINA SELENIUM ISCHEMIA-REPERFUSION APOPTOSIS pre-treatment POST-TREATMENT OXIDATIVE stress
• 年，卷（期）: 2015,（2）
• 页码范围: 263-268
• 页数: 6页
• 分类号: R774.1

摘要

AIM: To investigate the effects of selenium in rat retinal ischemia reperfusion(IR) model and compare pretreatment and post-treatment use.METHODS: Selenium pre-treatment group(n =8) was treated with intraperitoneal(i.p.) selenium 0.5 mg/kg for7 d and terminated 24 h after the IR injury. Selenium posttreatment group( n = 8) was treated with i. p. selenium0.5 mg/kg for 7d after the IR injury with termination at the end of the 7d period. Sham group(n =8) received i.p.saline injections identical to the selenium volume for 7d with termination 24 h after the IR injury. Control group(n =8) received no intervention. Main outcome measures were retina superoxide dismutase(SOD), glutathione(GSH),total antioxidant status(TAS), malondialdehyde(MDA),DNA fragmentation levels, and immunohistological apoptosis evaluation.RESULTS: Compared to the Sham group, selenium pre-treatment had a statistical difference in all parameters except SOD. Post-treatment selenium also resulted in statistical differences in all parameters except the MDA levels. When comparing selenium groups, the pre-treatment selenium group had a statistically higher success in reduction of markers of cell damage such as MDA and DNA fragmentation. In contrast, the post-selenium treatment group had resulted in statisticallyhigher levels of GSH. Histologically both selenium groups succeeded to limit retinal thickening and apoptosis. Pre-treatment use was statistically more successful in decreasing apoptosis in ganglion cell layer compared to post-treatment use.CONCLUSION: Selenium was successful in retinal protection in IR injuries. Pre-treatment efficacy was superior in terms of prevention of tissue damage and apoptosis.