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摘要:
In Vitro production of swine embryos is a valuable tool to generate clones and genetically modified pigs during a short period of time. However, the efficiency of the existing methods is extremely low and the oocyte quality and quantity represent important obstacles on the success of in Vitro production of embryos. Therefore, the aim of this study was to compare the in Vitro maturation, fertilization and subsequent embryo development rates of oocytes recovered by ovary slicing or follicular aspiration. The oocyte recovery rate (grade 1 COC/ovary) was higher (p = 0.0083) in the slicing group when compared to the aspiration group. No differences were observed between groups regarding in Vitro maturation and early cleavage rates. A higher percentage of oocytes recovered by follicular aspiration reached the blastocyst stage after IVF when compared to the ovary slicing method (p = 0.0395). However, no difference on blastocyst cell number was observed. Although the recovery of oocytes using the slicing technique yielded more grade 1 oocytes per ovary than the aspiration method, the number of oocytes that reached the blastocyst stage after IVF by the slicing method was lower when compared with oocytes obtained by aspiration, as observed by lower blastocyst rates. In conclusion, the follicular aspiration is the method of choice for porcine in Vitro embryo production.
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篇名 Effect of Oocyte Recovery Techniques on <i>in Vitro</i>Production of Swine Embryos
来源期刊 动物科学期刊(英文) 学科 医学
关键词 OOCYTE RECOVERY OOCYTE Maturation HSP70 EMBRYO Development SWINE
年,卷(期) 2015,(4) 所属期刊栏目
研究方向 页码范围 467-473
页数 7页 分类号 R73
字数 语种
DOI
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OOCYTE
RECOVERY
OOCYTE
Maturation
HSP70
EMBRYO
Development
SWINE
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动物科学期刊(英文)
季刊
2161-7597
武汉市江夏区汤逊湖北路38号光谷总部空间
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381
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