Anthrax lethal toxin suppresses high glucose induced VEGF over secretion through a post-translational mechanism

• 作者: Ping Xie Qing-Huai Liu Song-Tao Yuan Wei-Wei Zhang Xin Wang
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: angiogenesis diabetic RETINOPATHY retinal PIGMENTED EPITHELIUM vascular endothelial growth factor ANTHRAX LETHAL toxin
• 年，卷（期）: 2015,（3）
• 页码范围: 453-458
• 页数: 6页
• 分类号: R587.2

摘要

AIM: To prove anthrax lethal toxin(Le Tx) blocks the mitogen activated protein kinases(MAPKs) activation by degrading the MAPK/ERK kinases(MEKs) to suppress vascular endothelial growth factor(VEGF) secretion.METHODS: Human adult retinal pigmented epithelium(ARPE) cells were cultured and treated with normal glucose, high glucose or high glucose with Le Tx for additional 24, 48 or 72 h for viable cell count. Total RNA from the ARPE was isolated for reverse transcription polymerase chain reaction(RT-PCR). The conditioned medium of ARPE cells treated in different group for 48 h was filtered and diluted to detect the concentration of VEGF by enzyme-linked immunosorbant assays.Evaluate the role of MEK/MAPK pathway in the secretion of VEGF by immunoblotting. RESULTS: In this study, we proved high glucose induced activation of the MAPK extracellular signal-regulated kinase(ERK1/2) and p38 in the ARPE cell line was blocked by anthrax Le Tx. Le Tx also inhibited high glucose induced ARPE cell over proliferation.CONCLUSION: Le Tx suppressed high glucose induced VEGF over secretion in the ARPE cells, mainly through a post-translational mechanism.