<i>In Vitro</i>Propagation of Grapevine Cultivars with Potential for South of Brazil

• 作者: Aike Anneliese Kretzchmar Jean Carlos Bettoni Jo?o Peterson Pereira Gardin Juliana Aparecida Souza Murilo Dalla Costa
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: IAC 571-6 Poloskei Muskotaly In Vitro CULTURE VITIS CULTURE Medium
• 年，卷（期）: 2015,（11）
• 页码范围: 1806-1815
• 页数: 10页
• 分类号: R73

摘要

The micropropation is an important biotechnological tool for obtaining and maintaining mother vine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grape genotypes with potential for Southern Brazil. Vine nodal segments were cultured in five culture medium formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of IAC 571-6 rootstock and cv. Poloskei Muskotaly through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of IAC 571-6 and P?l?skei Muskotaly. Considering all the variables, the culture medium Roubelakis showed the best growth rates and development for shoots and roots, and in vitro multiplication rate for IAC 571-6 and Poloskei Muskotaly grape varieties.