Cannabinoids Production by Hairy Root Cultures of <i>Cannabis sativa</i>L.

• 作者: Oliver Kayser Sayed Farag
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: TETRAHYDROCANNABINOL CANNABINOIDS Callus HAIRY Root CANNABIS sativa
• 年，卷（期）: 2015,（11）
• 页码范围: 1874-1884
• 页数: 11页
• 分类号: R28

摘要

Tetrahydrocannabinol (THC) derivatives are used clinically as analgesic, anti-inflammatory, appetite stimulant, anti-emetic and anti-tumor cannabinoids. THC and its related compounds are at present obtained by extraction from intact Cannabis plants or chemical synthesis, but plant cell cultures may be an alternative source of production. In the present study, hairy root cultures of C. sativa (Cannabaceae) were induced by incubation of aseptically grown callus culture with solid B5 medium supplemented with 4 mg/l naphthaleneacetic acid in darkness at 25°C. Hairy root growth profiles in shake flask, increased periodically during 35 days of growth cycle. The cannabinoid contents produced in minor levels and remained below 2.0 μg/g dry weight. The contents of can-nabinoid were analyzed by liquid chromatography and confirmed by mass spectrometry.