Colonization of Rice Roots by a Green Fluorescent Protein-Tagged Isolate of Ustilaginoidea virens

• 作者: Aiqing Feng Jianxiong Li Luoye Li Mebeaselassie Andargie
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: ENDOPHYTES Plant-Fungal Interactions RHIZOSPHERE Root COLONIZATION Ustilaginoidea virens
• 年，卷（期）: 2015,（14）
• 页码范围: 2272-2279
• 页数: 8页
• 分类号: R73

摘要

The fungus U. virens is the causal agent of rice false smut disease. The green fluorescent protein (GFP) was used to mark this fungus in order to visualize and analyze the colonization and infection processes in vivo. Using epifluorescence microscopy colonization and infection on rice roots were visualized in vivo. After inoculation for 2 to 15 d, it was observed that the conidia and their germ-tubes had penetrated into epidermis of young roots. The hyphae were found inside the root xylem 18 d after inoculation. Generally, the transformed fungus colonized the rhizosphere, the cortex as well as the vascular tissues with symptoms of root necrosis observed. The results of this work show that U. virens colonize not only rice panicles but also the roots.