Rapid Determination of Three Kinds of Microcystins in Environmental Water Samples by Disk SPE-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

• 作者: Zhang Fuhai Zhang Min Zhao Bin
• 来源期刊: 气象与环境研究：英文版
• 学科: 地球科学
• 关键词: DISK SOLID-PHASE extraction COLUMN Ultra performance liquid chromatography-tandem mass SPECTROMETRY Environmental water sample MICROCYSTINS Lake Chao China
• 年，卷（期）: 2016,（5）
• 页码范围: 62-64
• 页数: 3页
• 分类号: P

摘要

A method of rapidly detecting three kinds of microcystins (MCs) in environmental water samples by using disk SPE - ultra high performance liquid chromatography -tandem mass spectrometry (UPLC -MS /MS) was established. Firstly, environmental water samples were extracted by disk SPE column (C 18) , and three kinds of MCs were separated by Waters BEH C18 chromatographic column with acetonitrile -0 .2 % formic acid solution as the mobile phase. After the gradient elution separation, the external standard method was used for quantitative and qualitative analysis under MRM of UPLC - MS/MS. The results showed that the three kinds of MCs in the range of 0.05 -10ug/L showed good linear relation, and the correlation coefficients were higher than 0.999 4, while the method detection limit was 0.04 ng/L. Under 0.1 , 1 , and 5 ug/L standard addition for the same environmental sample, the average recovery was 82.8% -1 0 8 .8 % , and the relative standard deviation of determination results was2.1% - 10 .1% (n=6) . This method is rapid, sensitive and accurate, so it can be effectively applied in the monitoring of MCs in environmental water samples.