4-51 Sensitizing Glioma Cells to Heavy Ions by Genistein is Related to the Expression of DNA-PKcs

• 作者: Li Qiang Liu Xiongxiong
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报：英文版
• 学科: 物理学
• 年，卷（期）: 2016,（1）
• 页码范围: 176-176
• 页数: 1页
• 分类号: O4

摘要

The previous studies have reported that DNA-dependent protein kinase catalytic subunit(DNA-PKcs)plays a major role in radiosensitivity of cancer cells[1;2].We used colony formation assays to investigate the effects of genistein on isogenic cell lines expressing different levels of DNA-PKcs after exposure to heavy ions.As shown in Fig.1,genistein increased the radiosensitivity of DNA-PKcs positive(M059K)but not negative(M059J)glioma cells to heavy ions.To further determine whether sensitizing glioma cells to heavy ions by genistein is related to DNA-PKcs,we treated cells with Nu7026,a specific inhibitor of DNA-PK,and siRNA assays to interfere the expression of DNA-PKcs,both of which increased the radiosentivity of DNA-PKcs proficient cancer cells(M059K)as almost the same level as the genistein-treated group.