Nitric oxide and tumour necrosis factor alpha in the process of pseudoexfoliation glaucoma

• 作者: Katarina B.Janicijevic Nemanja S.Zdravkovic Sladjana M.Pavlovic Tatjana S.Sarenac Vulovic Vladimir LJ.Jakovljevic
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: ascorbic acid NITRIC oxide PSEUDOEXFOLIATION TUMOUR NECROSIS factor alpha
• 年，卷（期）: 2016,（8）
• 页码范围: 1138-1142
• 页数: 0页
• 分类号: R775

摘要

AIM: To establish the role of nitric oxide(NO), ascorbic acid and tumour necrosis factor α(TNF-α) in the pathogenesis of pseudoexfoliation glaucoma(XFG).METHODS: Our study included 120 patients who were referred for cataract surgery. All patients were divided into four groups according to clinical findings: XFG,early and late pseudoexfoliation syndrome(XFS), and cataract(without pseudoexfoliation). Serum and aqueous humour levels of the ascorbic acid, NO and TNF-α were measured. The concentrations of the ascorbic acid and NO were measured by an appropriate spectrophotometric method. Enzyme-linked immunosorbent assay(ELISA)was used to determine TNF-α level.RESULTS: Aqueous humour concentration of ascorbic acid was significantly lower in patients with late XFS(0.61 ±0.11 mmol/L) and XFG(0.48 ±0.15 mmol/L)compared to patients with early XFS(0.9 ±0.15 mmol/L)and cataract(1.16 ±0.22 mmol/L), while there was no difference in serum concentration in all examined groups.Aqueous humour concentration of NO was significantly higher in patients with XFG(77.7 ±11.4 μmol/L) compared to patients with early XFS(50.27 ±9.34 μmol/L) and cataract(49.77 ±7.1 μmol/L), while serum concentration was increased in the early stage of XFS(73.26±8.29 μmol/L).Aqueous humour level of proinflammatory cytokine TNF-αwas increased in patients with XFS(early 460.04±18.32 pg/mL;late 502.42±53.23 pg/mL) and XFG(510.34±43.07 pg/mL),while there was no difference in serum level in all examined groups of patients.CONCLUSION: Reduced ascorbic acid and elevated NO and inflammation related cytokine TNF-α level in aqueous humour of the patients with developed XFG suggest that oxidative stress induces local inflammation.