Studies on Trans-Generational Transcriptional Silencing of <i>cry</i>1<i>Ac</i>Gene in Tobacco Transgenics

• 作者: Kamlesh Kumar Soni Madhurima Kahali Pradeep Kumar Burma
• 来源期刊: 美国分子生物学期刊(英文)
• 学科: 医学
• 关键词: CRY1AC Transcriptional SILENCING Bt Crops GENE SILENCING
• 年，卷（期）: 2017,（1）
• 页码范围: 1-10
• 页数: 10页
• 分类号: R73

摘要

Developing transgenics that express high levels of Cry1Ac protein, and at the same time, are phenotypically normal, has not been an easy task to achieve. It has been routinely observed that most of the transgenic plants that survive, show no or extremely low levels of Cry1Ac protein. However, all of these plants do express the selectable marker, nptII gene. In the present study, we record an interesting observation of how one of the genes (cry1Ac) on a single T-DNA fragment is selectively silenced, keeping the expression of the other gene (nptII) intact. Further, this silenced state is inherited.