Generation of genetically modified olfactory ensheathing cells that secrete VEGF: Potential application in cell-based treatment of leukoaraiosis

• 作者: Chuanqiang Qu Qinjian Sun Yifeng Du Yuanyuan Zhao
• 来源期刊: 临床转化神经医学(英文版)
• 学科: 医学
• 年，卷（期）: 2017,（2）
• 页码范围: 104-110
• 页数: 7页
• 分类号: R733.7

摘要

Objective: We aimed to provide an alternative cell source for cell therapy in leukoaraiosis(LA). Methods: Olfactory ensheathing cells(OECs) from the olfactory bulb were isolated,cultured, and purified. Next, the lentivirus carrying human VEGF165 gene was constructed and transfected into OECs. Results: The proliferative capacity of primary OECs was strong. OECs were infected with different multiplicity of infection. The expression level of VEGF was confirmed by real-time PCR with specific primers for GAPDH and VEGF, indicating that the genetically engineered OECs-VEGF produced VEGF with functional activity. Conclusions: Our data showed that these engineered OECs-VEGF highly express functional VEGF and retain the characteristics of astrocytes and Schwann cells,providing an alternative cell source for cell therapy in LA.