Down-Regulation of GKN1 in Gastric Cancer Is Not Associated with the RUNX3 Expression

• 作者: Antonella Federico Antonello Niglio Chiara Stella Di Stadio Emilia Rippa Filomena Altieri Giuseppina Miselli Maurizio De Palma Paolo Arcari
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: Gastrokine 1 GASTRIC Cancer EPIGENETICS RUNX3 Tumor SUPPRESSOR
• 年，卷（期）: 2017,（11）
• 页码范围: 80-90
• 页数: 11页
• 分类号: R73

摘要

Gastrokine 1 (GKN1) is a gastric mucosal protein highly expressed and secreted in normal individuals but during Helicobacter pylori infection or in gastric carcinogenesis it is strongly down-regulated or totally absent. In gastric cancer, the GKN1 gene is silenced through an epigenetic mechanism most likely mediated by a transcription factor that promotes on GKN1 promoter the activity of the enzymes SUV39H1 and HDACs. Because RUNX3 is a potential candidate in the regulation of molecular carcinogenesis process of stomach cancers, we tried to assess if RUNX3 could be involved in GKN1 down-regulation in GC. 17 paired of non-tumoral and tumoral surgical specimens from patients with gastric cancer were analyzed for GKN1 and RUNX3 by Western blotting and chromatin immunoprecipitation (Chip) assays. The overall results indicated that RUNX3 expression was not associated with the down-regulation of GKN1. The expression levels of RUNX3 in non-tumoral and tumoral samples suggest that RUNX3 does not act as a tumor suppressor but that it might play a complex oncogenic role in gastric cancer cells.