With rice as experimental material,the multi-strain solid-state fermentation method was used to study the preparation technology of taurine,and the analysis of variance was employed to determine the optimal process of taurine preparation as follows: fermentation time(72h); amount of cysteinyl acid( 2. 5 g),amount of methionine( 2. 5 g); ratio of Bacillus subtilis YS-45 to yeast S-78 to Aspergillus niger PL-39( 1 ∶ 2 ∶ 1). Under the optimal process,the taurine content reached 117. 8 mg/g.