Callogenesis of Cork Oak (<i>Quercus suber</i>L.) through <i>In Vitro</i>Culture of Nodes and Internodes

• 作者: Ahmed Lamarti Brahim El Bouzdoudi Mohammed L’bachir El Kbiach Rabah Sa?di Safaa Rahmouni Zineb Nejjar El Ansari
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Cork OAK QUERCUS suber L. NODES INTERNODES Callogenesis
• 年，卷（期）: mgzwxqkyw_2017,（8）
• 页码范围: 1801-1819
• 页数: 19页
• 分类号: R73

摘要

The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting;small white embryos appear on the surface of calluses.