Reduced Sensitivity of <i>Campomanesia adamantium</i>(Cambess.) O. Berg Seeds to Desiccation: Effects of Polyethylene Glycol and Abscisic Acid

• 作者: Daiane Mugnol Dresch Silvana De Paula Quint?o Scalon Tathiana Elisa Masetto Tatiane Sanches Jeromini
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Abscisic Acid OSMOTIC Conditioning Polyethylene Glycol Water Stress
• 年，卷（期）: 2017,（10）
• 页码范围: 2501-2515
• 页数: 15页
• 分类号: R73

摘要

The Campomanesia adamantium is a threatened species from Brazil Savannah which seeds are desiccation-sensitive and do not withstand storage. This study aimed to reduce the sensitivity of Campomanesia adamantium seeds to desiccation using polyethylene glycol (PEG) and abscisic acid (ABA). Initially, seeds were subjected to PEG (0, -1.48, and -2.04 MPa) with or without ABA (100 μM) during 120 h, followed fast drying (silica gel) or slow drying (laboratory environment), at 20%, 15%, and 10% moisture content. In the second experiment, the seeds were PEG treated (-1.48 MPa) which provided the best results in the first experiment;the seeds were then subjected to different incubation times in PEG (30, 60, 90, or 120 h) and ABA (0, 10ˉ3, 10ˉ4, and 10ˉ5 μM), following the seeds were fast dried at 15% moisture content. The slow drying should be avoided, even in seeds previously subjected to osmotic conditioning with or without ABA. Seeds submitted to PEG treatment (-1.48 MPa/120h) without ABA and PEG (-1.48 MPa) with 10ˉ3 or 10ˉ4 μM of ABA (90 h), followed by fast drying at 15% moisture content showed reduction of desiccation sensitivity and high germination and vigor when compared to the other treatments.