Anti-Tubercular Activities and Molecular Characterization of Salivary Extract of Leech (<i>Hirudo medicinalis</i>) against <i>Mycobacterium tuberculosis</i>

• 作者: Elisha Baba Hausatu Babayi Israel Kayode Olayemi Kasim Izebe Labake Ajoke Fadipe Oladosun Oluwale Peter Praise Olufunmilola Ojo
• 来源期刊: 结核病研究(英文)
• 学科: 医学
• 关键词: GC-MS MYCOBACTERIUM tuberculosis LEECH SALIVARY EXTRACT
• 年，卷（期）: jhbyjyw_2018,（1）
• 页码范围: 1-9
• 页数: 9页
• 分类号: R73

摘要

Leech salivary extract (LSE) was screened for the anti-tubercular activity against Mycobacterium tuberculosis using microbroth dilution method. The bioactive components in LSE were determined using Shimadzu GC-MS QP2010 SE. The LSE exhibited bactericidal effects on M. tuberculosis at 50% (v/v). The result of the GC-MS analysis of LSE revealed seventeen (17) bioactive components. The major fatty acid components were oleic acid (33.9%) and palmitic acid also known as hexadecanoic acid (22.6%). Other bioactive components detected in appreciable amounts in LSE include, 4-bromobutyric acid (16.86%), 6,17-Octadiene-1-ol acetate (9.78%) and octahydro-1,4,9,9- tetramethyl (5.21%). The anti-tubercular activity of the LSE was attributed to these bioactive components. The results obtained suggest that leech salivary extract could be used in treating tuberculosis.