The production of maxima and minima by the superposition of two or more light signals provides fundamental support for the wave nature of light. This result is based on the study of wave interference phenomena which remains the only approach to explain the production of those maxima and minima. In a system that is prepared to work with only one photon at a time, any detector can signal only one or zero. In 1986, a rigorously controlled experiment was designed by Grangier, G. Roger, and A. Aspect, [Europhys Lett. 1(4), p. 173, 1986] that guaranteed a single-photon beam. The explanation of the experimental results implied the interference of the wave function of a single-photon with itself. Thus, the explanation of interference that is accepted for an ensemble of photons was assumed to be valid for a single photon. In this study, we prepare a Mach-Zehnder interferometer using the same type of beam splitters used by Grangier et al. to test the assumption mentioned above. Our results allow us to explain the results of Grangier et al. because of the interaction between light and the beam splitters. Our results also verify that their wave interpretation of the results is not valid. Here, we present the essential findings of the extensive experimental evidence that supports our ideas.