The present study involves estimation of open channel flow parameters having different bed materials invoking data of Gradual Varied Flow (GVF). Use of GVF data facilitates estimation of flow parameters. The necessary data base was generated by conducting laboratory. In the present study, the efficacy of the Genetic Algorithm (GA) optimization technique is assessed in estimation of open channel flow parameters from the collected experimental data. Computer codes are developed to obtain optimal flow parameters Optimization Technique. Applicability, adequacy and robustness of the developed code are tested using sets of theoretical data generated by experimental work. A simulation model was developed to compute GVF depths at preselected discrete sections for given downstream head and discharge rate. This model is linked to an optimizer to estimate optimal value of decision variables. The proposed model is employed to a set of laboratory data for three bed materials. Application of proposed model reveals that optimal value of fitting parameter ranges from 1.42 to 1.48 as the material gets finer and optimal decision variable ranges from 0.015 to 0.024. The optimal estimates of Manning’s n of three different bed conditions of experimental channel appear to be higher than the corresponding reported/Strickler’s estimates.