In Search of Regulators of <i>LeSPL-CNR</i>by South-Western Blotting and Yeast One-Hybrid Library Screening System

• 作者: Bishun Ye Ting Zhou Tongfei Lai Xiaohong Wang Ying Wang
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Solanum lycopersicum LeSPL-CNR 286 bp Region South-Western Blotting Yeast One-Hybrid
• 年，卷（期）: 2018,（5）
• 页码范围: 1037-1050
• 页数: 14页
• 分类号: R73

摘要

LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR, which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.