Morphology and Thermal Characteristics of Polysaccharide Extracted by Ultrasound from <i>Ophiopogon japonicas</i>

• 作者: Dapeng Hao Miao Wang Qi Ding Tianzhu Zhang Wenfei Zhang Xiaomei Wang
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Ultrasonic Extraction POLYSACCHARIDE Thermal Analysis Environmental SCANNING Electron MICROSCOPY (ESEM)
• 年，卷（期）: mgzwxqkyw_2018,（8）
• 页码范围: 1699-1707
• 页数: 9页
• 分类号: R73

摘要

The crude polysaccharide UPOJ was extracted by ultrasound from Ophiopogon japonicas and purified by DEAE-cellulose52 column and Sephadex G-150 gel column. Purified polysaccharide UPOJ-DS was obtained. The thermal characteristics of UPOJ-DS were studied by using the thermal analysis system Q1000DSC LNCS FACS Q600SDT. The heating rate was 10°C/min and the test temperature was 20°C ~ 800°C. The results showed that there were three large mass losses in the process of heating, in which the maximum weight loss rate was 66.68% at 220°C ~ 330°C and the decomposition reaction took place in the polysaccharide itself, which indicated that the composition, water content, intermolecular interaction and aggregation behavior of UPOJ-DS were affected by temperature. The surface morphology of UPOJ-DS was observed by environmental scanning electron microscope (ESEM). The results indicated that the polysaccharide molecules were lamellar or clastic, with smooth surface, strong intermolecular interaction and close binding.