miR-23b promotes cutaneous wound healing through inhibition of the inflammatory responses by targeting ASK1

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: wound healing miR-23b inflammation keratinocytes ASK1
• 年，卷（期）: 2018,（11）
• 页码范围: 1104-1113
• 页数: 10页
• 语种: 英文
• DOI: 10.1093/abbs/gmy109

摘要

Wound healing is a complicated event that develops in three overlapping phases:inflammatory,proliferative,and remodeling.MicroRNAs (miRNAs) have been proved to play an important role in the healing process of skin trauma,and alteration of specific miRNA expression during different phases may be associated with abnormal wound healing.In this study,we determined the variation of miR-23b expression after trauma in normal mice and in cultured cells exposed to lipopolysaccharide.We further demonstrated that excessive miR-23b could significantly accelerate wound healing in vivo.Up-regulation of miR-23b decreases infiltration of inflammatory cells,as evidenced by pathologic staining.Meanwhile,miR-23b could significantly inhibit the expression of pro-inflammatory cytokines,including TNF-α,IL-1β,IL-6,and Ccl2,and significantly increase antiinflammatory factor IL-10.Furthermore,miR-23b could also promote α-SMA expression in a fiber pattern and increase the expression of Col1a1 and Col3a1.Importantly,we also showed that miR23b could inhibit inflammation to promote wound healing by targeting apoptotic signal-regulating kinase 1 (ASK1).Notably,knockdown of ASK1 could reduce inflammation factor expression in vitro.Together,our data reveal that miR-23b is a potent therapeutic agent for cutaneous wound healing that shortens the period of inflammatory responses and promotes keratinocyte migration for the re-epithelialization of wound sites.