Molecular Characterization of Rotavirus Strains Circulating in Enugu Nigeria: 2011 to 2016

• 作者: A. Isiaka B. N. Tagbo B. O. Edelu C. Azubuike C. B. Eke C. Benjamin-Puja C. Chukwubike C. Odume E. O. Obidike G. Armah H. U. Okafor ICH UNTH Enugu Rotavirus Group J. M. Mphahlele J. M. Mwenda K. M. Yusuf L. Namadi M. L. Seheri M. U. Okwesili N. Ogude N. Umezinne O. Udemba R. Njoku R. O. Nnani S. K. Ezebilo U. C. Ozumba V. Okafor V. Osaro
• 来源期刊: 疫苗(英文)
• 学科: 医学
• 关键词: ROTAVIRUS Molecular Characterization Children GENOTYPE
• 年，卷（期）: 2019,（1）
• 页码范围: 22-36
• 页数: 15页
• 分类号: R73

摘要

Rotavirus gastroenteritis is a major public health concern globally, estimated to cause 215,000 deaths among children < 5 years of age in 2013;with majority of mortality occurring in developing countries. In 2013, it was estimated that Nigeria was the second country with the highest number of rotavirus deaths. Monitoring of circulating rotavirus strains in Enugu, Nigeria is part of on-going rotavirus surveillance before the introduction of rotavirus vaccination. A total of 2694 stool samples were collected from enrolled under 5 years old children with diarrhoea between January 2011 and December 2016 and tested the virus using an antigen enzyme immunoassay. Randomly selected rotavirus positive samples were further characterized by rotavirus genotype methods to identify the G and P types circulating during the study period. Rotavirus was detected in 1242 (46%) of the 2694 samples collected over the six years period. Of these, 867 were randomly selected for genotyping. G and P types could be assigned for 832 samples (96%), while 31 (3.6%) could only be assigned either genotype G or P (partially typed) and 4 (0.4%) could not be assigned genotype G and P (untypeable). The most common G-genotypes detected during the entire study period were G12, G1 and G3 accounting for 27.6%, 21.0% and 16.3% respectively. Mixed G and P-genotypes were commonly detected. Ninety-one of the samples, representing 10.8% (91/839) had mixed G-genotype whilst 130 of the samples representing 15.2% (130/852) had mixed P-genotype. The most common P-genotypes detected were P[8], P[6] and P[4] representing 38.3%, 35.4% and 9.1% respectively. The predominant strain detected was G12P[8] (22.3%) followed by G3P[6] (14.5%), G1P[8] (9.2%) and G1P[6] (8.0%). These data are useful for making an informed decision about the introduction of rotavirus vaccine into the national routine immunization program and to monitor the impact of the vaccine post licensure.