Development of Disposable Single-Use Biosensor for Fructosyl Valine and Glycated Hemoglobin A1c

• 作者: Jessica Leng Sean Liu Theonalyn C. Aquino
• 来源期刊: 传感技术(英文)
• 学科: 工学
• 关键词: SCREEN Printed BIOSENSOR Glycated HEMOGLOBIN A1C Diabetes
• 年，卷（期）: 2019,（4）
• 页码范围: 45-53
• 页数: 9页
• 分类号: TP2

摘要

A novel amperometric biosensor prototype was fabricated using screen printing technique. The disposable single-use strips were made from conductive carbon ink and modified with fructosyl amino acid oxidase. The electrodes and conducting paths were made solely with carbon ink and characterized by conductivity and cyclic voltammetry. The biosensor showed high current output, large linearity, and effectiveness for fructosyl valine as well as human blood samples. Amperometric studies were carried out using both fructosyl valine and human blood samples. With 5 uL sample volume, the biosensor showed strong amperometric response with good linearity for a wide range (0 to 8 mM). Diabetic and healthy blood samples showed sufficient difference in their amperometric responses that correlate well with their different hemoglobin A1c levels. These results demonstrate the feasibility of using this type of inexpensive single-use biosensor strips as the basis for determining hemoglobin A1c levels for diabetic patients.