Hepatitis C&B still the major problem in hemodialysis patients and the challenge continues in dialysis centers to prevent spread among patients and workers,the worldwide reported incidence of anti-HC antibody in dialysis center was range from 10.5%-24% yearly and the prevalence range 5-85%.Also,HBs-antigen had the same problem but with less incidence and prevalence.For the purpose of evaluating the seroprevalence and seroconversion of anti-HC antibody and HBs-antigen in Haemodialysis centers at Baghdad required action a retrospective incidence study from Nov.2015 to Jan.2016 on maintenance HD patients at hemodialysis centers in Al-kindi,Al-Karama and Baghdad Hospitals.The data obtained from patients records of hospitals and we relied on laboratory test results for ELISA to determine the seroconversion and seroprevalence of anti-HC-antibody and HBs-Antigen.The study found the total prevalence of seropositive anti-HCV antibody in three centers were 251(28%),the seropositive patients of HBs-Ag were 14(2%)While the total seroconversion was 196(21%)anti-HCV antibody and for HBs-Ag 4(0.4%);so there was serious risk of nosocomial infection particularly for hepatitis C in the hemodialysis centers in Baghdad and AL-kindi while the problem with HBV was under control at same time The differences of seroconversion and seroprevalence of anti-HC antibody and HBs-antigen between the hospitals in the study may indicate to the level of infection control was applied in hospital.